p akt2 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
p akt2
P Akt2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+akt2/Phospho-Akt2+(Ser474)+Rabbit+mAb/pm41714926-106-23-26
Average 95 stars, based on 187 article reviews
P Akt2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+akt2/Phospho-Akt2+(Ser474)+Rabbit+mAb/pm41714926-106-23-26
Average 95 stars, based on 187 article reviews
p akt2 - by Bioz Stars,
2026-09
95/100 stars
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Incubation:Article Title: Oxidized Low-Density Lipoprotein Accumulation in Macrophages Impairs Lipopolysaccharide-Induced Activation of AKT2, ATP Citrate Lyase, Acetyl-Coenzyme A Production, and Inflammatory Gene H3K27 Acetylation. Article Snippet: Membranes were blocked with 5% skim milk nonfat powder or 3% BSA (BioShop, no. ALB003) in TBST for 1 h at room temperature. .. Membranes were incubated with primary Abs overnight as follows: antiactin (Sigma-Aldrich, no. A2066), anti p-AKT (Ser473) (CST, no. 4060), anti p-AKT (Thr308) (CST, no. 4056), AKT (CST, no. 4685), anti p-PRAS40 (Thr246) (CST, no. 13175), anti-PRAS40 (CST, no. 2691), anti p-S6K (Thr389) (CST, no. 9234), anti-S6K (CST, no. 9202), anti p-S6 (Ser235/236) (CST, no. 4858), anti-S6 (CST, no. 2217), anti p-4EBP1 (Ser65) (CST, no. 9451), anti-4EBP1 (CST, no. 9644), anti p-GSK3a/b (Ser21/9) (CST, no. 9327), anti p-GSK3a/b (CST, no. 5676), anti p-AKT1 (Ser473) (CST, no. 9018), anti-AKT1 (CST, no. 2938), anti p-AKT2 (Ser474) (CST, no. 8599), Article Title: LRRC8 complexes are adenosine nucleotide release channels regulating platelet activation and arterial thrombosis Article Snippet: Gels were transferred onto Immun-Blot PVDF Membranes (#1620177, Bio-Rad) in tris/glycine buffer (#1610771, Bio-Rad) for at 100 V for 1.5 h at 4°C. .. Membranes were then blocked in 5% bovine serum albumin (#A-420-10, GoldBio, St. Louis, MO, USA), or milk, in tris-buffered saline with Tween (TBS-T; 0.2 M Tris, 1.37 M NaCl, 0.2% Tween-20, pH 7.4) at room temperature for 1 h. Membranes were subsequently incubated with rabbit polyclonal anti-LRRC8A against the epitope QRTKSRIEQGIVDRSE (Pacific Immunology, Ramona, CA, USA), anti-LRRC8B*, anti-LRRC8C (#21601-1-AP, Proteintech, Rosemont, IL, USA), anti-LRRC8D*, anti-LRRC8E*, anti-AKT1 (#2938, Cell Signaling Technology, Danvers, MA, USA), anti-pAKT1 Ser473 (#9018, Cell Signaling Technology), Saline:Article Title: LRRC8 complexes are adenosine nucleotide release channels regulating platelet activation and arterial thrombosis Article Snippet: Gels were transferred onto Immun-Blot PVDF Membranes (#1620177, Bio-Rad) in tris/glycine buffer (#1610771, Bio-Rad) for at 100 V for 1.5 h at 4°C. .. Membranes were then blocked in 5% bovine serum albumin (#A-420-10, GoldBio, St. Louis, MO, USA), or milk, in tris-buffered saline with Tween (TBS-T; 0.2 M Tris, 1.37 M NaCl, 0.2% Tween-20, pH 7.4) at room temperature for 1 h. Membranes were subsequently incubated with rabbit polyclonal anti-LRRC8A against the epitope QRTKSRIEQGIVDRSE (Pacific Immunology, Ramona, CA, USA), anti-LRRC8B*, anti-LRRC8C (#21601-1-AP, Proteintech, Rosemont, IL, USA), anti-LRRC8D*, anti-LRRC8E*, anti-AKT1 (#2938, Cell Signaling Technology, Danvers, MA, USA), anti-pAKT1 Ser473 (#9018, Cell Signaling Technology), Concentration Assay:Article Title: LRRC8 complexes are adenosine nucleotide release channels regulating platelet activation and arterial thrombosis Article Snippet: Gels were transferred onto Immun-Blot PVDF Membranes (#1620177, Bio-Rad) in tris/glycine buffer (#1610771, Bio-Rad) for at 100 V for 1.5 h at 4°C. .. Membranes were then blocked in 5% bovine serum albumin (#A-420-10, GoldBio, St. Louis, MO, USA), or milk, in tris-buffered saline with Tween (TBS-T; 0.2 M Tris, 1.37 M NaCl, 0.2% Tween-20, pH 7.4) at room temperature for 1 h. Membranes were subsequently incubated with rabbit polyclonal anti-LRRC8A against the epitope QRTKSRIEQGIVDRSE (Pacific Immunology, Ramona, CA, USA), anti-LRRC8B*, anti-LRRC8C (#21601-1-AP, Proteintech, Rosemont, IL, USA), anti-LRRC8D*, anti-LRRC8E*, anti-AKT1 (#2938, Cell Signaling Technology, Danvers, MA, USA), anti-pAKT1 Ser473 (#9018, Cell Signaling Technology), other:Article Title: Phosphorylation of plectin by Akt3 promotes triple-negative breast cancer cell invasive migration Article Snippet: Anti-phospho-Akt substrate (pAkt-motif) (9614S), anti-phospho-Akt Ser473 (4060L), anti-Akt (4691S), anti-phospho-PRAS40 Thr246 (2997S), anti-PRAS40 (2691S), anti-phospho-GSK3β (9336S), anti-GSK3β (9315), anti-Akt1 (2938S), anti-Akt2 (3063S), anti-Akt3 (8018S), and Article Title: Pleiotropic tumor suppressive functions of PTEN missense mutations during gliomagenesis Article Snippet: |
